In class, we examine slides that are often prepared with the H&E staining process. While we have gone over H&E stains before, I am choosing to us this weeks blog as a focus on the preparation of these tissue slides in order to refresh myself about the process of H&E stains and how different tissue types respond to this type of staining.
According to a journal entry in Basic Methods of Microscopy, H&E stains have been used to years. They are highly responsive to the different components of body tissue and display them well. Hematoxylin stains stains tissues blue by way of staining nucleic acids while eosin stains tissues pink by way of staining proteins. The journal entry was very detailed with how the staining process occurs and how each chemical reacts with the tissue.
My main focus during investigation and research of H&E stains was to determine why they are more preferred over other methods of staining. Based on LabCE, H&E stains are most common because they provide more depth to an image. The The structures can be differentiated from one another based on the different colors that come from the stains. This helps those studying the tissues determine whether it is abnormal tissue or not. The entry where this question was found was very straightforward with stating why H&E stains are more effective, however, it only mentioned how it is beneficial in pathology.
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